Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collected from different areas and estimate its genome size. We use corn as the internal reference plant and rice as the internal reference plant to correct the data, and control the CV value below 5.0% to ensure the accuracy of the results. The results showed that the genome size of P. kingianum was about 6.90±0.41 pg. Moreover, high-quality results have CV values of approximately 1.0%–2.0%, and the conventional results have CV values of approximately 3.0%. The average coefficient of variation in this study is 2.5%-3.5%; therefore, the genome size of the three populations in this study has high reliability. In a word, this study provides the first estimate of the genome size of P. kingianum. In the future, these results can be used to establish sequence data and provide a basis for determining the whole genome of P. kingianum. Moreover, these results provide a basis for selecting research materials for future work on P. kingianum genetic evolution and molecular breeding.
| Published in | Journal of Plant Sciences (Volume 10, Issue 6) |
| DOI | 10.11648/j.jps.20221006.11 |
| Page(s) | 203-208 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Flow Cytometry (FCM), Genome Size, Polygonatum Kingianum
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APA Style
Xiao-Lei Chen, Kun Cong, Zhe Xu, Hua-Li Qian, Hao Chen, et al. (2022). Polygonatum Kingianum Genome Size Estimation by Flow Cytometry. Journal of Plant Sciences, 10(6), 203-208. https://doi.org/10.11648/j.jps.20221006.11
ACS Style
Xiao-Lei Chen; Kun Cong; Zhe Xu; Hua-Li Qian; Hao Chen, et al. Polygonatum Kingianum Genome Size Estimation by Flow Cytometry. J. Plant Sci. 2022, 10(6), 203-208. doi: 10.11648/j.jps.20221006.11
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Download@article{10.11648/j.jps.20221006.11,
author = {Xiao-Lei Chen and Kun Cong and Zhe Xu and Hua-Li Qian and Hao Chen and Jian-Yun Su and Lei Zhang and Jia-Hong Dong and Peng-Zhang Ji},
title = {Polygonatum Kingianum Genome Size Estimation by Flow Cytometry},
journal = {Journal of Plant Sciences},
volume = {10},
number = {6},
pages = {203-208},
doi = {10.11648/j.jps.20221006.11},
url = {https://doi.org/10.11648/j.jps.20221006.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20221006.11},
abstract = {Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collected from different areas and estimate its genome size. We use corn as the internal reference plant and rice as the internal reference plant to correct the data, and control the CV value below 5.0% to ensure the accuracy of the results. The results showed that the genome size of P. kingianum was about 6.90±0.41 pg. Moreover, high-quality results have CV values of approximately 1.0%–2.0%, and the conventional results have CV values of approximately 3.0%. The average coefficient of variation in this study is 2.5%-3.5%; therefore, the genome size of the three populations in this study has high reliability. In a word, this study provides the first estimate of the genome size of P. kingianum. In the future, these results can be used to establish sequence data and provide a basis for determining the whole genome of P. kingianum. Moreover, these results provide a basis for selecting research materials for future work on P. kingianum genetic evolution and molecular breeding.},
year = {2022}
}
TY - JOUR T1 - Polygonatum Kingianum Genome Size Estimation by Flow Cytometry AU - Xiao-Lei Chen AU - Kun Cong AU - Zhe Xu AU - Hua-Li Qian AU - Hao Chen AU - Jian-Yun Su AU - Lei Zhang AU - Jia-Hong Dong AU - Peng-Zhang Ji Y1 - 2022/11/04 PY - 2022 N1 - https://doi.org/10.11648/j.jps.20221006.11 DO - 10.11648/j.jps.20221006.11 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 203 EP - 208 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20221006.11 AB - Polygonatum kingianum, an herb of Polygonatum in Liliaceae, is a kind of Chinese herbal medicine with homology of medicine and food. Polygonatum kingianum has extensive scientific research, but its genome size is seldom studied. In order to enrich the genome data of P. kingianum, this study was based on flow cytometry to detect P. kingianum collected from different areas and estimate its genome size. We use corn as the internal reference plant and rice as the internal reference plant to correct the data, and control the CV value below 5.0% to ensure the accuracy of the results. The results showed that the genome size of P. kingianum was about 6.90±0.41 pg. Moreover, high-quality results have CV values of approximately 1.0%–2.0%, and the conventional results have CV values of approximately 3.0%. The average coefficient of variation in this study is 2.5%-3.5%; therefore, the genome size of the three populations in this study has high reliability. In a word, this study provides the first estimate of the genome size of P. kingianum. In the future, these results can be used to establish sequence data and provide a basis for determining the whole genome of P. kingianum. Moreover, these results provide a basis for selecting research materials for future work on P. kingianum genetic evolution and molecular breeding. VL - 10 IS - 6 ER -
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