The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon.
| Published in | Journal of Plant Sciences (Volume 9, Issue 5) |
| DOI | 10.11648/j.jps.20210905.14 |
| Page(s) | 253-261 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Agrobacterium, miR393a, Transformation, Tuberous Begonia, Water Stress Tolerance
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APA Style
Tongsu Ho, Haksong Pak, Sokjun Ri, Kang Kim, Namhyok Mun. (2021). Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. Journal of Plant Sciences, 9(5), 253-261. https://doi.org/10.11648/j.jps.20210905.14
ACS Style
Tongsu Ho; Haksong Pak; Sokjun Ri; Kang Kim; Namhyok Mun. Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. J. Plant Sci. 2021, 9(5), 253-261. doi: 10.11648/j.jps.20210905.14
AMA Style
Tongsu Ho, Haksong Pak, Sokjun Ri, Kang Kim, Namhyok Mun. Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation. J Plant Sci. 2021;9(5):253-261. doi: 10.11648/j.jps.20210905.14
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Download@article{10.11648/j.jps.20210905.14,
author = {Tongsu Ho and Haksong Pak and Sokjun Ri and Kang Kim and Namhyok Mun},
title = {Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation},
journal = {Journal of Plant Sciences},
volume = {9},
number = {5},
pages = {253-261},
doi = {10.11648/j.jps.20210905.14},
url = {https://doi.org/10.11648/j.jps.20210905.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20210905.14},
abstract = {The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon.},
year = {2021}
}
TY - JOUR T1 - Improvement of Water Stress Tolerance of Tuberous Begonia (Begonia×tuberhybrida) by OsmiR393a Gene Transformation AU - Tongsu Ho AU - Haksong Pak AU - Sokjun Ri AU - Kang Kim AU - Namhyok Mun Y1 - 2021/09/29 PY - 2021 N1 - https://doi.org/10.11648/j.jps.20210905.14 DO - 10.11648/j.jps.20210905.14 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 253 EP - 261 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20210905.14 AB - The genus Begonia is greatly affected by abiotic stresses, which lead to losses in greenhouse production and flower longevity. Obtaining more-tolerant plants is a very important breeding goal in ornamentals. To achieve abiotic tolerant Begonia×tuberhybrida, OsmiR393a, a miRNA involved in the tolerance of plants to abiotic stresses, was introduced using Agrobacterium-mediated transformation. Explants were cultured on modified MS medium supplemented with 1.0 mg/L BA, 0.5 mg/L IAA, and 5 mg/L hygromycin for transgenic selection and regeneration. Stable integration of the transgene was verified in putatively transformed plants by PCR screening. When fresh flower longevity was evaluated by the period from bloom to wilting of 3 petals, flower longevity of transgenic line was 8 d longer than control. Furthermore, 15 days after stress treatment, petals of control plants wilted, whereas transgenic lines remained. This results shows that expressing OsmiR393a could extend only flower longevity without affecting flower type under water stress in Begonia×tuberhybrida. In addition, the transgenic plants displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to water stress. These results suggest OsmiR393a may improve water stress tolerance of Begonia×tuberhybrida by regulating different pathways in response to the stress conditions and miR393a is conservatized between monocotyledon and dicotyledon. VL - 9 IS - 5 ER -
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