Genetic Diversity Analysis of Ethiopian Yam (<i>Dioscorea</i> spp) from Different Geographic Origin Using Simple Sequence Repeat

Atnafua Bekele; Endashaw Bekele

doi:doi:10.11648/j.jps.20210903.14

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Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat

Atnafua Bekele, Endashaw Bekele

Published in Journal of Plant Sciences (Volume 9, Issue 3)

Received: 16 September 2020 Accepted: 26 May 2021 Published: 21 June 2021

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Abstract

Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.

Published in	Journal of Plant Sciences (Volume 9, Issue 3)
DOI	10.11648/j.jps.20210903.14
Page(s)	96-106
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Yam, Genetic Diversity, Geographic Distance, SSR

References

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[17]	Obidiegwu, J., Kolesnikova-Allen M., Ene-obong, E., Muoneke, C., and Asiedu, A., (2009). SSR markers reveal diversity in Guinea yam (D. cayenensis/D. rotundata) core set. African Journal of Biotechnology Vol. 8 (12), pp. 2730-2739.
[18]	Obidiegwu, E., Asiedu, R., Ene-Obong, E., Muoneke, C, and Kolesnikova, M, (2009). Genetic characterization of some water yam (Dioscorea alata L.) accessions in West Africa with simple sequenc repeats. Journal of Food & Agriculture.

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Atnafua Bekele, Endashaw Bekele. (2021). Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. Journal of Plant Sciences, 9(3), 96-106. https://doi.org/10.11648/j.jps.20210903.14

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ACS Style

Atnafua Bekele; Endashaw Bekele. Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. J. Plant Sci. 2021, 9(3), 96-106. doi: 10.11648/j.jps.20210903.14

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AMA Style

Atnafua Bekele, Endashaw Bekele. Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. J Plant Sci. 2021;9(3):96-106. doi: 10.11648/j.jps.20210903.14

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@article{10.11648/j.jps.20210903.14,
  author = {Atnafua Bekele and Endashaw Bekele},
  title = {Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat},
  journal = {Journal of Plant Sciences},
  volume = {9},
  number = {3},
  pages = {96-106},
  doi = {10.11648/j.jps.20210903.14},
  url = {https://doi.org/10.11648/j.jps.20210903.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20210903.14},
  abstract = {Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.},
 year = {2021}
}

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TY  - JOUR
T1  - Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat
AU  - Atnafua Bekele
AU  - Endashaw Bekele
Y1  - 2021/06/21
PY  - 2021
N1  - https://doi.org/10.11648/j.jps.20210903.14
DO  - 10.11648/j.jps.20210903.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 96
EP  - 106
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20210903.14
AB  - Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.
VL  - 9
IS  - 3
ER  -

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Author Information

Atnafua Bekele

South Agricultural Research Institute, Hawassa Agricultural Research Centre, Hawassa, Ethiopia
Endashaw Bekele

Addis Ababa Universities, Microbial, Cellular and Molecular Biology Department, Addis Ababa, Ethiopia

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Plain Text BibTeX RIS

APA Style

Atnafua Bekele, Endashaw Bekele. (2021). Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. Journal of Plant Sciences, 9(3), 96-106. https://doi.org/10.11648/j.jps.20210903.14

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ACS Style

Atnafua Bekele; Endashaw Bekele. Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. J. Plant Sci. 2021, 9(3), 96-106. doi: 10.11648/j.jps.20210903.14

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AMA Style

Atnafua Bekele, Endashaw Bekele. Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat. J Plant Sci. 2021;9(3):96-106. doi: 10.11648/j.jps.20210903.14

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@article{10.11648/j.jps.20210903.14,
  author = {Atnafua Bekele and Endashaw Bekele},
  title = {Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat},
  journal = {Journal of Plant Sciences},
  volume = {9},
  number = {3},
  pages = {96-106},
  doi = {10.11648/j.jps.20210903.14},
  url = {https://doi.org/10.11648/j.jps.20210903.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20210903.14},
  abstract = {Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.},
 year = {2021}
}

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TY  - JOUR
T1  - Genetic Diversity Analysis of Ethiopian Yam (Dioscorea spp) from Different Geographic Origin Using Simple Sequence Repeat
AU  - Atnafua Bekele
AU  - Endashaw Bekele
Y1  - 2021/06/21
PY  - 2021
N1  - https://doi.org/10.11648/j.jps.20210903.14
DO  - 10.11648/j.jps.20210903.14
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 96
EP  - 106
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20210903.14
AB  - Yam (Dioscorea spp L.) is one of root and tuber crops grown in Ethiopia as source of food and income. It is cultivated mainly in south, southwest, and western part of the country. Two hundred ten yam accessions from ten different geographic origins of major growing areas of the country were used in this study. The sprouted tubers of some accessions were received from research centers where others were directly collected from farmers’ fields during early March, 2010. The collected yam genotypes were planted in complete randomized block design at two research sites (namely at Hawasa and Wonago) which are found under South Agricultural Research Institute in the end of April 2010. All important cultural practices such as staking, weeding and irrigation were done starting from planting till harvesting. Yam leaf samples with of different origin were observed separately using twenty SSR markers. A similarity matrix derived UPGMA cluster analysis based on geographical sites showed nine distinct clustering groups which indicated presence of relationship between genetic distances to that of geographical distance in most of clustering groups. These cluster grouping is supported by Analysis of Molecular Variance (AMOVA) that indicated presence of significance genetic variation of 210 yam accessions within and among ten collection sites.
VL  - 9
IS  - 3
ER  -

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